Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus.
Identifieur interne : 000557 ( Main/Exploration ); précédent : 000556; suivant : 000558Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus.
Auteurs : Neha Lohia [Inde] ; Manoj Baranwal [Inde]Source :
- Viral immunology [ 1557-8976 ] ; 2015.
Descripteurs français
- KwdFr :
- Antigènes d'histocompatibilité de classe I (), Antigènes d'histocompatibilité de classe I (métabolisme), Antigènes d'histocompatibilité de classe II (), Antigènes d'histocompatibilité de classe II (métabolisme), Biologie informatique, Déterminants antigéniques des lymphocytes T (immunologie), Humains, Liaison aux protéines, Protéines de la matrice virale (), Protéines de la matrice virale (immunologie), Protéines de la matrice virale (métabolisme), Simulation de docking moléculaire, Sous-type H1N1 du virus de la grippe A (immunologie), Séquence conservée.
- MESH :
- immunologie : Déterminants antigéniques des lymphocytes T, Protéines de la matrice virale, Sous-type H1N1 du virus de la grippe A.
- métabolisme : Antigènes d'histocompatibilité de classe I, Antigènes d'histocompatibilité de classe II, Protéines de la matrice virale.
- Antigènes d'histocompatibilité de classe I, Antigènes d'histocompatibilité de classe II, Biologie informatique, Humains, Liaison aux protéines, Protéines de la matrice virale, Simulation de docking moléculaire, Séquence conservée.
English descriptors
- KwdEn :
- Computational Biology, Conserved Sequence, Epitopes, T-Lymphocyte (immunology), Histocompatibility Antigens Class I (chemistry), Histocompatibility Antigens Class I (metabolism), Histocompatibility Antigens Class II (chemistry), Histocompatibility Antigens Class II (metabolism), Humans, Influenza A Virus, H1N1 Subtype (immunology), Molecular Docking Simulation, Protein Binding, Viral Matrix Proteins (chemistry), Viral Matrix Proteins (immunology), Viral Matrix Proteins (metabolism).
- MESH :
- chemical , chemistry : Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Viral Matrix Proteins.
- chemical , immunology : Epitopes, T-Lymphocyte, Viral Matrix Proteins.
- chemical , metabolism : Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Viral Matrix Proteins.
- immunology : Influenza A Virus, H1N1 Subtype.
- Computational Biology, Conserved Sequence, Humans, Molecular Docking Simulation, Protein Binding.
Abstract
Cell mediated immune response plays a key role in combating viral infection and thus identification of new vaccine targets manifesting T cell mediated response may serve as an ideal approach for influenza vaccine. The present study involves the application of an immunoinformatics-based consensus approach for epitope prediction (three epitope prediction tools each for CD4+ and CD8+ T cell epitopes) and molecular docking to identify peptide sequences containing T cell epitopes using the conserved sequences from all the Matrix 1 protein sequences of H1N1 virus available until April 2015. Three peptides comprising CD4+ and CD8+ T cell epitopes were obtained, which were not exactly reported in earlier studies. Population coverage study of these multi-epitope peptides revealed that they are capable of inducing a potent immune response belonging to individuals from different populations and ethnicity distributed around the globe. Conservation study with other subtypes of influenza virus infecting humans (H2N2, H5N1, H7N9, and H3N2) revealed that these three peptides were conserved (>90%), with 100% identity in most of these strains. Hence, these peptides can impart immunity against H1N1 as well as other subtypes of influenza virus. A molecular docking study of the predicted peptides with class I and II human leukocyte antigen (HLA) molecules has shown that the majority of them have comparable binding energies to that of native peptides. Hence, these peptides from Matrix 1 protein of H1N1 appear to be promising candidates for universal vaccine design.
DOI: 10.1089/vim.2015.0060
PubMed: 26398199
Affiliations:
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Le document en format XML
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xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Thapar University , Patiala</wicri:regionArea><wicri:noRegion>Patiala</wicri:noRegion></affiliation></author></analytic><series><title level="j">Viral immunology</title><idno type="eISSN">1557-8976</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Computational Biology</term><term>Conserved Sequence</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Histocompatibility Antigens Class I (chemistry)</term><term>Histocompatibility Antigens Class I (metabolism)</term><term>Histocompatibility Antigens Class II (chemistry)</term><term>Histocompatibility Antigens Class II (metabolism)</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (immunology)</term><term>Molecular Docking Simulation</term><term>Protein Binding</term><term>Viral Matrix Proteins 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Class I</term><term>Histocompatibility Antigens Class II</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Histocompatibility Antigens Class I</term><term>Histocompatibility Antigens Class II</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes T</term><term>Protéines de la matrice virale</term><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes d'histocompatibilité de classe I</term><term>Antigènes d'histocompatibilité de classe II</term><term>Protéines de la matrice virale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Computational Biology</term><term>Conserved Sequence</term><term>Humans</term><term>Molecular Docking Simulation</term><term>Protein Binding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Antigènes d'histocompatibilité de classe I</term><term>Antigènes d'histocompatibilité de classe II</term><term>Biologie informatique</term><term>Humains</term><term>Liaison aux protéines</term><term>Protéines de la matrice virale</term><term>Simulation de docking moléculaire</term><term>Séquence conservée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cell mediated immune response plays a key role in combating viral infection and thus identification of new vaccine targets manifesting T cell mediated response may serve as an ideal approach for influenza vaccine. The present study involves the application of an immunoinformatics-based consensus approach for epitope prediction (three epitope prediction tools each for CD4+ and CD8+ T cell epitopes) and molecular docking to identify peptide sequences containing T cell epitopes using the conserved sequences from all the Matrix 1 protein sequences of H1N1 virus available until April 2015. Three peptides comprising CD4+ and CD8+ T cell epitopes were obtained, which were not exactly reported in earlier studies. Population coverage study of these multi-epitope peptides revealed that they are capable of inducing a potent immune response belonging to individuals from different populations and ethnicity distributed around the globe. Conservation study with other subtypes of influenza virus infecting humans (H2N2, H5N1, H7N9, and H3N2) revealed that these three peptides were conserved (>90%), with 100% identity in most of these strains. Hence, these peptides can impart immunity against H1N1 as well as other subtypes of influenza virus. A molecular docking study of the predicted peptides with class I and II human leukocyte antigen (HLA) molecules has shown that the majority of them have comparable binding energies to that of native peptides. Hence, these peptides from Matrix 1 protein of H1N1 appear to be promising candidates for universal vaccine design. </div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Lohia, Neha" sort="Lohia, Neha" uniqKey="Lohia N" first="Neha" last="Lohia">Neha Lohia</name></noRegion><name sortKey="Baranwal, Manoj" sort="Baranwal, Manoj" uniqKey="Baranwal M" first="Manoj" last="Baranwal">Manoj Baranwal</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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